Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis

Table 3

Validation of six miRNAs’ expression levels by qRT-PCR assays in the discovery ( pairs) and validation ( pairs) sets.

miRNAs	log2 expression levels		Fold change^*	P
	Mean (SD)
	Tumor	Nontumor
Discovery set
miR-10a-5p	−2.77 (1.03)	−2.52 (0.91)	−1.19
miR-125b-5p	−1.45 (1.34)	−1.13 (1.05)	−1.25
miR-199a-3p	−2.39 (2.16)	−1.67 (1.27)	−1.64
miR-18a-5p	−7.20 (1.58)	−8.49 (0.84)	2.45
miR-182	−8.63 (1.93)	−10.62 (1.89)	3.97
miR-1180	−8.21 (1.82)	−9.12 (1.06)	1.88
Validation set
miR-10a-5p	−2.58 (1.17)	−2.04 (1.01)	−1.45
miR-125b-5p	−1.44 (1.27)	−0.85 (1.10)	−1.51
miR-199a-3p	−2.69 (2.30)	−1.32 (1.47)	−2.58
miR-18a-5p	−8.09 (1.31)	−8.44 (0.95)	1.27
miR-182	−10.04 (2.03)	−11.33 (1.73)	2.45
miR-1180	−8.63 (1.60)	−9.28 (1.15)	1.57

Fold change >0 indicates overexpression; <0 indicates decreased expression.