Research Article
Prognostic Impact of mRNA Expression Levels of HER1–4 (ERBB1–4) in Patients with Locally Advanced Rectal Cancer
Table 1
Primer/probe sets used for amplification of the target and reference genes.
| Gene | Oligo ID | Oligo sequences 5′-3′ | Label probe | Amplicon length (nts) | Assay location | PCR efficacy in % |
| MKI67 | Forward | CGAGACGCCTGGTTACTATCAA | CY5-BHQ2 | 108 | c. ex 2-3 NM_002417 | 98 | Reverse | GGATACGGATGTCACATTCAATACC | Probe | ACGGTCCCCACTTTCCCCTGAGC |
| CALM2 | Forward | GAGCGAGCTGAGTGGTTGTG | YY-BHQ1 | 72 | c. ex 1-2 NM_001743 | 99.3 | Reverse | AGTCAGTTGGTCAGCCATGCT | Probe | TCGCGTCTCGGAAACCGGTAGC |
| EGFR (ERBB1) | Forward | CGCAAGTGTAAGAAGTGCGAA | FAM-BHQ1 | 93 | NM_201283 | 89.1 | Reverse | CGTAGCATTTATGGAGAGTGAGTCT | Probe | CCTTGCCGCAAAGTGTGTAACGGAAT |
| ERBB2 (HER2) | Forward | TCTGGACGTGCCAGTGTGAA | CY5-BHQ2 | 61 | M11730_gen | 97.2 | Reverse | CCTGCTCCCTGAGGACACAT | Probe | AGGCCAAGTCCGCAGAAGCCCT |
| ERBB3 (HER3) | Forward | CGGTTATGTCATGCCAGATACAC | ROX-BHQ2 | 81 | NM_001982 | 96 | Reverse | GAACTGAGACCCACTGAAGAAAGG | Probe | CTCAAAGGTACTCCCTCCTCCCGGG |
| ERBB4 (HER4) | Forward | GAGGCTGCTCAGGACCTAAGG | ATTO-BHQ1 | 75 | NM_005235 | 92 | Reverse | GAGTAACACATGCTCCACTGTCATT | Probe | CACAGACTGCTTTGCCTGCATGAATTTC |
|
|
CY5 = cyanine dye 5, BHQ = black hole quencher; FAM = 6-carboxyfluorescein; ROX = carboxy-X-rhodamine; ATTO = fluorescent dye (ATTO-TEC GmbH, Siegen, Germany).
|