DAB2IP Downregulation Enhances the Proliferation and Metastasis of Human Gastric Cancer Cells by Derepressing the ERK1/2 Pathway

Figure 5

DAB2IP knockdown promotes gastric cancer cell growth and metastasis in a ERK1/2 signaling pathway manner. (a) Western blotting analysis of the p-ERK1/2 and ERK expression in wild-type cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). Tubulin as a loading control. (b) Western blot was used to detect the level of p-ERK1/2, ERK1/2, E-cadherin, and vimentin in the presence or absence of U0126 (20 μM). (c) Colony formation assays were performed in wild-type SGC7901 cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP) with or without U0126 (20 μM). Representative photographs are presented (left; magnification, ×1), and the colonies containing above 100 cells were counted (right). The bands were quantified and presented as the mean ± SEM of three independent experiments. (d) Migration assays were performed in wild-type SGC7901 cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP) with or without U0126 (20 μM). Representative photographs are presented (left; magnification, ×200), and the relative number of migratory cells (right) were counted. The bands were quantified and presented as the mean ± SEM of three independent experiments. Statistical significance was determined by a two-tailed, unpaired Student t-test. .

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