Figure 3: Biliary epithelial senescence in PBC. BECs in small bile ducts involved in CNSDC show histological features of senescence, such as cytoplasmic eosinophilia, cellular and nuclear enlargement, and uneven nuclear spacing (a). SA-β-gal activity is detected in BECs in PBC (b). Senescent markers, and , were expressed in BECs in damaged small bile ducts in PBC (c, d). Immunostaining for and . Original magnification, 400.