Hepatitis Research and Treatment / 2014 / Article / Tab 1 / Research Article
Interleukin-16 Gene Polymorphisms Are Considerable Host Genetic Factors for Patients’ Susceptibility to Chronic Hepatitis B Infection Table 1 PCR and RFLP information for the SNPs of interleukin-16 gene.
Polymorphism Primer PCR (bp) Restriction enzyme (incubation temp °C) Restricted fragments’ size (bp) rs4072111 C/T F: 5′-CACTGTGATCCCGGTCCAGTC-3′ R: 5′-TTCAGGTACAAACCCAGCCAG C-3′ 164 BsmAI (55) C: 164 T: 140 + 24 rs11556218 T/G F: 5′-GCTCAGGTTCACAGAGTGTTTCCATA-3′ R: 5′-TGTGACAATCACAGCTTGCCTG-3′ 171 Nde I (37) T: 171 G: 147 + 24 rs4778889 T/C F: 5′-CTCCACACTCAAAGCCTTTTGTTCCTAT A-3′ R: 5′-CCATGTCAAAACGGTAGCCTCAAGC-3′ 280 AhdI (37) T: 280 C: 246 + 34
The underlined base in the forward primer is different from that of the original sequence and serves as the introduction of a recognition site for the restriction enzyme AhdI.