Experimental design using the VX2 rabbit model of secondary liver tumors. The VX2 cell line was implanted in the muscle of F1 rabbits 4 weeks prior to tumor procurement. The experimental group was formed by rabbits where 1 mm³ tumor grafts were implanted in their liver. The Sham group consisted of rabbits that underwent similar operation, but 1 mm³ pieces of gelfoam were implanted in their liver. Enrichment of body water was achieved at 5% of total body water, and blood samples were taking at days −13, −12, and −11 prior to tumor implantation and at days +11, +12, and +13 after tumor implantation.