Small-for-Size Liver Transplantation Increases Pulmonary Injury in Rats: Prevention by NIM811

Figure 1

NIM811 protects against graft injury and promotes liver regeneration after transplantation of small-for-size liver grafts. Full-size and reduced-size rat livers were transplanted, as described in Section 2. In some experiments, NIM811 (NIM, 5 μM) was added to the storage and poststorage lactated Ringer’s rinse solutions. In (a) and (b), liver grafts were harvested at 38 h after transplantation for H&E staining ((a), bar is 100 μm) or BrdU immunohistochemistry ((b), bar is 50 μm). Representative images are shown. Arrows identify necrotic areas. Panels are as follow: 1st row, liver from a sham-operated rat; 2nd row, FSG (100%); 3rd row, HSG (50%); 4th row, QSG (25%); 5th row, QSG treated with NIM811. In (c), blood samples were collected at 38 h after transplantation for ALT measurement. In (d), proliferating cell nuclear antigen (PCNA) expression in liver tissue was detected by immunoblotting and quantified by densitometry. Values are means ± S.E.M. Group sizes were 4 per group: (a) 𝑃<0.05 versus sham operation; (b) 𝑃<0.05 versus FSG (100%); (c) 𝑃<0.05 versus HSG (50%); (d) 𝑃<0.05 versus QSG (25%).