Small-for-Size Liver Transplantation Increases Pulmonary Injury in Rats: Prevention by NIM811

Figure 3

NIM811 decreases lung inflammation after small-for-size liver transplantation. Full-size and reduced-size rat livers were transplanted, as described in Section 2. Lungs were harvested 38 h after transplantation. Alveolar septal thickness (a) was quantified by image analysis of 5 random alveolar septa per image from 10 random images per H&E-stained slide using a IPlab 3.7v software. ED1 positive cells (b) and myeloperoxidase (MPO) positive cells (c) after immunohistochemical staining were counted in 10 random fields per slide using a 40x objective lens. Sham: lungs from sham-operated rats; 100%: lungs from FSG recipients; 50%: lungs from HSG recipients; 25%: lungs from QSG recipients; 25% + NIM: lungs from recipients of QSG treated with NIM811. Values are means ± S.E.M. Group sizes were 4 per group: (a) 𝑃<0.05 versus sham operation; (b) 𝑃<0.05 versus FSG (100%); (c) 𝑃<0.05 versus HSG (50%); (d) 𝑃<0.05 versus QSG (25%).

(a)

(b)

(c)