Rapid Determination of Macrolide and Lincosamide Resistance in Group B Streptococcus Isolated from Vaginal-Rectal Swabs

Dela Cruz, Wilfred P.; Richardson, Joann Y.; Broestler, Judith M.; Thornton, Jennifer A.; Danaher, Patrick J.

doi:https://doi.org/10.1155/2007/46581

Infectious Diseases in Obstetrics and Gynecology

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Research Article | Open Access

Volume 2007 | Article ID 046581 | https://doi.org/10.1155/2007/46581

Rapid Determination of Macrolide and Lincosamide Resistance in Group B Streptococcus Isolated from Vaginal-Rectal Swabs

Wilfred P. Dela Cruz,¹Joann Y. Richardson,²Judith M. Broestler,³Jennifer A. Thornton,¹and Patrick J. Danaher⁴

Received23 Jan 2007

Accepted16 Apr 2007

Published26 Jun 2007

Abstract

Objective. Our objective was to assess the ability of real-time PCR to predict in vitro resistance in isolates of group B streptococcus (GBS). Methods. The first real-time PCR assays for the genes known to confer resistance to erythromycin and clindamycin in GBS were developed. Three hundred and forty clinical GBS isolates were assessed with these assays and compared with conventional disk diffusion. Results. The presence of an erythromycin ribosome methylation gene (ermB or ermTR variant A) predicted in vitro constitutive or inducible resistance to clindamycin with a sensitivity of 93% (95% CI 86%–97%), specificity of 90% (95% CI 85%–93%), positive predictive value of 76% (95% CI 67%–84%), and negative predictive value of 97% (95% CI 94%–99%). Conclusion. This rapid and simple assay can predict in vitro susceptibility to clindamycin within two hours of isolation as opposed to 18–24 hours via disk diffusion. The assay might also be used to screen large numbers of batched isolates to establish the prevalence of resistance in a given area.

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Copyright

Copyright © 2007 Wilfred P. Dela Cruz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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