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Figure 1: Subtilosin A immobilizes human spermatozoa in a dose-dependent manner. The percentage of motile spermatozoa in pooled whole semen was determined 30 seconds after mixing with subtilosin A, at different final concentrations, as indicated. All data were adjusted to a normal control motility of 70% and subjected to arcsine transformation before further analysis. Values are expressed as average % motility. Error bars are 90% confidence limits.