Research Article

Rapid, Simultaneous Detection of Clostridium sordellii and Clostridium perfringens in Archived Tissues by a Novel PCR-Based Microsphere Assay: Diagnostic Implications for Pregnancy-Associated Toxic Shock Syndrome Cases

Table 1

Oligonucleotide primers and probes used in the duplex microsphere assay.

PrimerSequence (5′-3′)Gene targetProduct size (bp)

Clostridium sordellii primers and probes
CSP09-F primer TGG  GAT  GAT  TGG  GAT  TAT  TCA GPhospholipase C of C. sordellii (Csp)176  bp
CSP09-BR primer TCA  GTT  CCT  GCA  TAT  TCA  TTG  TPhospholipase C of C. sordellii (Csp)
CSP09 probe AGA  AGC  GAT  AAA  AAA  TTC  TCA  APhospholipase C of C. sordellii (Csp)
Clostridium perfringens primers [17] and probes
PL3-F primer AAG  TTA  CCT  TTG  CTG  CAT  AAT  CCC Phospholipase C of C. perfringens (plc)283 bp
PL7-BR primer ATA  GAT  ACT  CCA  TAT  CAT  CCT  GCTPhospholipase C of C. perfringens (plc)
CP1 probe TTT  AGC  AAA  ACC  TCT  TGPhospholipase C of C. perfringens (plc)