CD8+CXCR5+ T Cells Regulate Pathology in the Genital Tract
Figure 3
Adoptive transfer of CD8+ cells reversed oviduct dilation late after C. muridarum infection in mice. (a) Donor and WT mice were infected with C. muridarum and the spleens were harvested 7 days later and lymphocytes were obtained by dissociating cells within the organs. Donor C57BL/6 WT mice were infected with C. muridarum and the spleens harvested 7 days after infection. CD8+ cells were purified by FACS sorting for expression of CD8. lymphocytes and WT-CD8+ were adoptively transferred (1 × 106) into mice as indicated. Genital tracts were harvested 7 weeks later and hemetoxylin and eosin staining was performed and scored. Oviducts were scored individually and were compared between recipient groups using Kruskal-Wallis test. Transfer groups are, WT donor into WT recipient; mice (12 oviducts), donor into recipient, mice (12 oviducts), and WT-CD8 donor into recipient, mice (4 oviducts). The bars indicate the mean ± SEM and *. (b) Recipient mice were vaginally swabbed 9 days after infection and all groups were compared by one-way ANOVA. There were no differences between groups. The bars indicate the mean IFU ± SEM.
