Research Article

Accuracy of Curable Sexually Transmitted Infections and Genital Mycoplasmas Screening by Multiplex Real-Time PCR Using a Self-Collected Veil among Adult Women in Sub-Saharan Africa

Table 4

Relevant selected examples of paired results obtained by gold standard clinician-collected endocervical swab and self-collected veil using the multiplex real-time PCR Allplex™ STI Essential Assay (Seegene). Participants #021, #036, and #233 gave similar profiles of bacteria detection by paired clinician-collected swab and self-collected veil. Participants #058 and #152 showed more detection by swab than by veil. Finally, participants #009, #041, #090, #126, #172, #173, #177, and #231 showed better bacteria detection by self-collected veil than clinician-collected swab. Positive result for a given bacteria is indicated by cross whereas negative result by white box. All swab and veil specimens were positive for β-globin internal control of the PCR Allplex™ STI Essential Assay (not shown). Group I corresponds to common curable sexually transmitted infections (Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis) and group II to commensal mycoplasmas (Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum).

SpecimensGenital microorganisms detection
Group IGroup II
C. trachomatisN. gonorrhoeaeM. genitaliumT. vaginalisM. hominisU. urealiticumU. parvum

#009
Veil++++
Swab+++

#021
Veil++
Swab++

#036
Veil+++
Swab+++

#041
Veil+
Swab

#058
Veil+
Swab+++

#090
Veil++
Swab

#126
Veil++
Swab+

#152
Veil
Swab++

#172
Veil+
Swab

#173
Veil+++
Swab++

#177
Veil++
Swab+

#231
Veil++
Swab

#233
Veil+++
Swab+++