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International Journal of Agronomy
Volume 2012 (2012), Article ID 198960, 8 pages
http://dx.doi.org/10.1155/2012/198960
Research Article

Genetic Transformation of Common Bean (Phaseolus vulgaris L.) with the Gus Color Marker, the Bar Herbicide Resistance, and the Barley (Hordeum vulgare) HVA1 Drought Tolerance Genes

Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA

Received 18 May 2012; Revised 19 July 2012; Accepted 29 July 2012

Academic Editor: Antonio M. De Ron

Copyright © 2012 Kingdom Kwapata et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Five common bean (Phaseolus vulgaris L.) varieties including “Condor,” “Matterhorn,” “Sedona,” “Olathe,” and “Montcalm” were genetically transformed via the Biolistic bombardment of the apical shoot meristem primordium. Transgenes included gus color marker which visually confirmed transgenic events, the bar herbicide resistance selectable marker used for in vitro selection of transgenic cultures and which confirmed Liberty herbicide resistant plants, and the barley (Hordeum vulgare) late embryogenesis abundant protein (HVA1) which conferred drought tolerance with a corresponding increase in root length of transgenic plants. Research presented here might assist in production of better P. vulgaris germplasm.