Table 1: Transient expression of GUS using different gene gun pressure (psi), DNA plasmid concentration and bombardment frequency for optimizing the Biolistic bombardment conditions for the pCATIF containing the gus gene.

Bombardment pressure (psi)Concentration of plasmid DNA (μg)Bombardment frequencyMean transformation percent

1.5 1
500 1.5 2
500 1.5 3
500 3 1
500 3 2
500 3 3
1000 1.5 1
1000 1.5 2
1000 1.5 3
1000 3 1
1000 3 2
1000 3 3
1100 1.5 1
1100 1.5 2
1100 1.5 3
1100 3 1
1100 3 2
1100 3 3