598163.fig.003a
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598163.fig.003b
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598163.fig.003c
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598163.fig.003d
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598163.fig.003e
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Figure 3: (a)  PCR analysis of the combination of HVA1-mtlD in T1 maize lines. (b) Northern blot hybridization of T1 HVA1 in HVA1-mtlD transgenic lines using the HVA1 probe. (c) Northern blot hybridization of T1 mtlD in the HVA1-mtlD transgenic lines using the mtlD probe. (d) Northern blot hybridization of T2 HVA1 in HVA1-mtlD transgenic lines using the HVA1 probe. (e) RCR analysis of the T3 HVA1-mtlD confirming the stability of both transgenes in the fourth (T3) progeny obtained from northern blot positive HVA1 and mtlD T2 line number 51-1.