Optimization of Molecularly Imprinted Polymer Method for Rapid Screening of 17β-Estradiol in Water by Fluorescence Quenching

Figure 4

Fluorescence emission intensity at 310 nm versus concentration of quencher: (a) 2.5 mg/mL E2-MIP particles in aqueous suspension quenched with AuNPs, (b) 3.5 ppm E2 aqueous solution quenched with AuNPs, (c) 2.5 mg/mL E2-bound NIP particles in aqueous suspension quenched with AuNPs, (d) 2.5 mg/mL E2-MIP particles in aqueous suspension going through two steps of fluorescence quenching, first with AuNPs and next with sodium nitrite (standard error bars, approximately three-times the size of each data point symbol, are omitted here for the sake of clarity).