Table 1: Effect of O2 reducing atmospheres on aflatoxins, lipids, microorganisms and consumers acceptance of packaged shelled Brazil nuts during storage.

Storage Total fungi count*(cfu·g−1)Aspergillus toxigenic strainsm.c.aAflatoxinsb (μg·kg−1)Lipid stability (mg·kg−1)cBrazil nuts sensory attributes (scores)d
Atmosphere Day(%)(diff.%)eAPCOFIRASROD

GROUP I—Controlf

Air

Initial1.83 × 104 (+) A. flavus; A. parasiticus h6.5<0.367.24 ± 0.9444444
Final2.69 × 107(+) A. flavus; A. parasiticus 8.1(+0.6%)<1.899.98 ± 1.6233232

Vacuum

Initial1.83 × 104(+) A. flavus; A. parasiticus 4.2<0.367.33 ± 0.4444444
Final0.70 × 10(+) A. flavus; A. parasiticus 4.2(no diff.)<0.367.24 ± 1.1444444

GROUP IIi

Air

11.83 × 104(+) A. flavus; A. parasiticus 6.515.007.33 ± 0.1444444
302.96 × 105(+) A. flavus; A. parasiticus 7.115.818.25 ± 0.2434333
606.30 × 106(+) A. flavus; A. parasiticus 7.1(+0.6%)16.859.24 ± 0.7333132

Vacuum

11.83 × 104(+) A. flavus; A. parasiticus 4.215.006.85 ± 0.5444444
300.56 × 10NGj4.214.887.24 ± 0.8444444
600.10 × 10NG4.2(−2.3%)14.957.24 ± 0.5455444

Ozone

1NGNG5.0<0.367.25 ± 1.2444444
30NGNG4.9<0.367.24 ± 0.6444444
60NGNG4.7(−1.8%)<0.367.84 ± 0.6454444

Ozone + vacuum

1NGNG3.1<0.366.25 ± 0.2444444
30NGNG3.3<0.367.04 ± 0.7444444
60NGNG3.0(−3.5%)<0.367.25 ± 0.5445444
Carbon dioxide

11.83 × 104(+) A. flavus; A. parasiticus 6.515.007.25 ± 0.2444444
30NGNG7.014.907.24 ± 1.1444444
60NGNG7.0(+0.5%)14.927.90 ± 0.6344443

Oxygen absorber pad

11.83 × 104(+) A. flavus; A. parasiticus 6.515.007.25 ± 2.2444444
302.6 × 10NG6.514.907.90 ± 1.7444444
60NGNG6.5(no diff.)15.008.20 ± 0.6434443

Oxygen  absorber pad  +  vacuum

11.8 × 104(+) A. flavus; A. parasiticus 4.015.007.00 ± 2.2444444
30NGNG3.915.017.24 ± 1.5444444
60NGNG4.3(−2.2%)14.997.24 ± 1.5435443

GROUP IIIk

Ozone

1NGNG5.6<0.367.56 ± 0.9444444
30NGNG5.4<0.367.94 ± 0.2444444
60NGNG5.2(−1.6%)<0.367.99 ± 0.5443444

Ozone + vacuum

1NGNG4.0<0.36 7.95 ± 0.4444444
30NGNG3.9<0.36 7.94 ± 1.2444444
60NGNG3.7(−3.2%)<0.368.54 ± 0.6434444

am.c.: moisture content; baflatoxin total: AFB1+AFB2+AFG1+AFG2 (method LOQ: 0.350 μg/kg); cin malondialdehyde; dvalues as mean scores of 18 individual panellists [AP: nut appearance; CO: color; FI: firmness; RA: rancid odour; SR: slicing resistant; OD: strange odor (5: like very much, 4: like, 3: neither like nor dislike, 2: dislike and 1: dislike very much)]; ediff: m.c. difference (+) increased or (−) reduction; fno aflatoxin spiked (nuts total AFL < method LOQ = 0.36 μg·kg−1); htoxigenic Aspergillus strains isolated in AFPA media; i15 μg·kg−1 AFLs spiked and 6.5% m.c.; jNG: no growth; kBrazil nuts naturally aflatoxin contaminated = 10.61 μg·kg−1 and 7.2% m.c. *The genera and species more often isolated from the Control Brazil nuts were Acremonium sp; A. ochraceus; A. nomius; Cladosporium sp.; P. corylophilum, and Rhizopus sp. followed by A. niger; A. parasiticus; A. versicolor, and P. crustosum.