Table 3: Validation of analytical method for BHA, BHT, and OMC by RP-HPLC-UV/Vis.

CompoundRetention time (minutes)Calibration equation RSD%LOD (mg/L)LOQ (mg/L)

BHA2.60y =19673x + 25790.9990.180.1960.593
BHT4.35y = 13410x − 55510.9990.170.1700.515
OMC4.95y = 95019x − 140040.9990.250.4781.448