Rapid Screening for Flavone C-Glycosides in the Leaves of Different Species of Bamboo and Simultaneous Quantitation of Four Marker Compounds by HPLC-UV/DAD

Table 4

Recoveries of isoorientin, isovitexin, orientin, and vitexin by the HPLC method.

Bamboo species	Compound	Original contents (μg/mL)	Spiked (μg/mL)	Amount found (μg/mL)	Recovery^a (%)	RSD (%)
Bambusa multiplex; cv. Silverstripe	Isoorientin	39.79	21.26	59.38	92.16	2.33
		39.79	42.52	81.80	98.80	1.11
		39.79	59.53	96.74	95.76	2.18
	Isovitexin	9.46	4.00	13.16	92.42	3.25
		9.46	8.00	17.45	99.92	0.81
		9.46	11.20	20.54	98.96	4.66
Phyllostachys heterocycla; cv. Pubesceus	Isoorientin	15.18	7.59	2.81	100.53	3.02
		15.18	15.18	29.72	95.76	5.01
		15.18	22.77	37.78	99.25	1.53
	Orientin	3.89	2.00	6.01	105.83	3.22
		3.89	4.00	7.90	100.17	5.25
		3.89	6.00	10.23	105.61	4.03
	Vitexin	0.96	0.50	1.41	89.33	7.20
		0.96	1.00	1.93	96.67	7.48
		0.96	1.50	2.29	88.44	4.90
	Isovitexin	4.02	2.00	6.12	105.00	4.23
		4.02	4.00	8.20	104.42	2.88
		4.02	6.00	10.24	103.61	4.00
Phyllostachys acuta	Isoorientin	20.77	11.00	31.07	93.61	1.10
		20.77	22.00	41.72	95.21	3.92
		20.77	33.00	52.71	96.79	3.98
	Orientin	4.30	2.00	6.33	101.50	5.55
		4.30	4.00	7.89	89.75	4.69
		4.30	6.00	10.11	96.83	3.48
	Isovitexin	2.84	2.50	5.07	89.07	6.13
		2.84	5.00	7.89	101.00	4.98
		2.84	7.50	9.73	91.87	3.53

^amean ± SD (𝑛=3).