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International Journal of Analytical Chemistry
Volume 2012 (2012), Article ID 260425, 10 pages
doi:10.1155/2012/260425
Review Article

Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract

Hiroyuki Tanaka,1 Osamu Morinaga,2 Takuhiro Uto,2 Shunsuke Fuji,3 Frederick Asare Aboagye,4 Nguyen Huu Tung,2 Xiao Wei Li,5 Waraporn Putalun,6 and Yukihiro Shoyama2

1Graduate School of Pharmaceutical Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-0855, Japan
2Faculty of Pharmaceutical Science, Nagasaki International University, 2827-7 Huis Ten Bosch, Sasebo 859-3298, Japan
3Faculty of Health Management, Nagasaki International University, 2827-7 Huis Ten Bosch, Sasebo 859-3298, Japan
4Phytochemistry Department, Centre for Scientific Research into Plant Medicine, University of Ghana, P.O. Box 73, Mampong-Akuapem, Ghana
5State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, No.38 Xue-yuan Road, Haidian District, Beijing 100191, China
6Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen 40002, Thailand

Received 30 August 2011; Accepted 18 October 2011

Academic Editor: Norberto Peporine Lopes

Copyright © 2012 Hiroyuki Tanaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. R. Sakata, Y. Shoyama, and H. Murakami, “Production of monoclonal antibodies and enzyme immunoassay for typical adenylate cyclase activator, forskolin,” Cytotechnology, vol. 16, no. 2, pp. 101–108, 1994. View at Scopus
  2. M. Ishiyama, Y. Shoyama, H. Murakami, and H. Shinohara, “Production of monoclonal antibodies and development of an ELISA for solamargine,” Cytotechnology, vol. 18, no. 3, pp. 153–158, 1996. View at Scopus
  3. L. Xuan, H. Tanaka, Y. Xu, and Y. Shoyama, “Preparation of monoclonal antibody against crocin and its characterization,” Cytotechnology, vol. 29, no. 1, pp. 65–70, 1999. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  4. H. Tanaka, Y. Goto, and Y. Shoyama, “Monoclonal antibody based enzyme immunoassay for marihuana (cannabinoid) compounds,” Journal of Immunoassay, vol. 17, no. 4, pp. 321–342, 1996. View at Scopus
  5. Y. Shoyama, T. Fukada, and H. Murakami, “Production of monoclonal antibodies and ELISA for thebaine and codeine,” Cytotechnology, vol. 19, no. 1, pp. 55–61, 1995. View at Scopus
  6. H. Tanaka, N. Fukuda, and Y. Shoyama, “Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization,” Cytotechnology, vol. 29, no. 2, pp. 115–120, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. N. Fukuda, H. Tanaka, and Y. Shoyama, “Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: monoclonal antibody for a ginseng saponin,” Cytotechnology, vol. 34, no. 3, pp. 197–204, 2000. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  8. J. S. Kim, H. Tanaka, and Y. Shoyama, “Immunoquantitative analysis for berberine and its related compounds using monoclonal antibodies in herbal medicines,” Analyst, vol. 129, no. 1, pp. 87–91, 2004. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  9. O. Morinaga, S. Nakajima, H. Tanaka, and Y. Shoyama, “Production of monoclonal antibodies against a major purgative component, sennoside B, their characterization and use in ELISA,” Analyst, vol. 126, no. 8, pp. 1372–1376, 2001. View at Publisher · View at Google Scholar · View at Scopus
  10. Z. Lu, O. Morinaga, H. Tanaka, and Y. Shoyama, “A quantitative ELISA using monoclonal antibody to survey paeoniflorin and albiflorin in crude drugs and traditional Chinese herbal medicines,” Biological and Pharmaceutical Bulletin, vol. 26, no. 6, pp. 862–866, 2003. View at Scopus
  11. H. Tanaka and Y. Shoyama, “Formation of a monoclonal antibody against glycyrrhizin and development of an ELISA,” Biological and Pharmaceutical Bulletin, vol. 21, no. 12, pp. 1391–1393, 1998. View at Scopus
  12. S. Shan, H. Tanaka, and Y. Shoyama, “Enzyme-linked immunosorbent assay for glycyrrhizin using anti-glycyrrhizin monoclonal antibody and an eastern blotting technique for glucuronides of glycyrrhetic acid,” Analytical Chemistry, vol. 73, no. 24, pp. 5784–5790, 2001. View at Publisher · View at Google Scholar · View at Scopus
  13. P. Loungratana, H. Tanaka, and Y. Shoyama, “Production of monoclonal antibody against ginkgolic acids in Ginkgo biloba Linn,” American Journal of Chinese Medicine, vol. 32, no. 1, pp. 33–48, 2004. View at Publisher · View at Google Scholar · View at Scopus
  14. K. Katsumi, K. Edakuni, O. Morinaga, H. Tanaka, and Y. Shoyama, “An enzyme-linked immunosorbent assay for aconitine-type alkaloids using an anti-aconitine monoclonal antibody,” Analytica Chimica Acta, vol. 616, no. 1, pp. 109–114, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  15. K. Kido, O. Morinaga, Y. Shoyama, and H. Tanaka, “Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody,” Talanta, vol. 77, no. 1, pp. 346–350, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  16. H. Tanaka, N. Fukuda, and Y. Shoyama, “Eastern blotting and immunoaffinity concentration using monoclonal antibody for ginseng saponins in the field of traditional Chinese medicines,” Journal of Agricultural and Food Chemistry, vol. 55, no. 10, pp. 3783–3787, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  17. I. Wengatz, R.-D. Schmid, S. Kreissig, et al., “Determination of the hapten density of immuno-conjugates by matrix-assisted UV laser desorption/ionization mass spectrometry,” Analytical Letters, vol. 25, pp. 1983–1997, 1992.
  18. Y. Shoyama, T. Fukada, T. Tanaka, A. Kusai, and K. Nojima, “Direct determination of opium alkaloid-bovine serum albumin conjugate by matrix-assisted laser desorption/ionization mass spectrometry,” Biological and Pharmaceutical Bulletin, vol. 16, no. 10, pp. 1051–1053, 1993. View at Scopus
  19. Y. Shoyama, R. Sakata, R. Isobe, H. Murakami, A. Kusai, and K. Nojima, “Direct determination of forskolin-bovine serum albumin conjugate by matrx-assisted laser desorption ionization mass spectrometry,” Organic Mass Spectrometry, vol. 28, pp. 987–988, 1993.
  20. Y. Goto, Y. Shima, S. Morimoto, et al., “Determination of tetrahydrocannabinolic acid-carrier protein conjugate by matrix-assisted laster desorption/Ionization mass spectrometry and antibody formation,” Organic Mass Spectrometry, vol. 29, pp. 668–671, 1994.
  21. The Society for Korean Ginseng, In Understanding of Korean Ginseng, The Society for Korean Ginseng, Seoul, Korea, 1995.
  22. I. Kitagawa, T. Taniyama, M. Yoshikawa, Y. Ikenishi, and Y. Nakagawa, “Chemical studies on crude drug processing. VI. (1)) Chemical structures of malonyl-Ginsenosides Rb1, Rb2, Rc, and Rd isolated from the root of Panax ginseng C.A. Meyer,” Chemical and Pharmaceutical Bulletin, vol. 37, no. 11, pp. 2961–2970, 1989. View at Scopus
  23. O. Morinaga, H. Tanaka, and Y. Shoyama, “Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re,” Journal of Chromatography B, vol. 830, no. 1, pp. 100–104, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  24. H. Tanaka, W. Putalun, C. Tsuzaki, and Y. Shoyama, “A simple determination of steroidal alkaloid glycosides by thin-layer chromatography immunostaining using monoclonal antibody against solamargine,” FEBS Letters, vol. 404, no. 2-3, pp. 279–282, 1997. View at Publisher · View at Google Scholar · View at Scopus
  25. N. Fukuda, S. Shan, H. Tanaka, and Y. Shoyama, “New staining methodology: eastern blotting for glycosides in the field of Kampo medicines,” Journal of Natural Medicines, vol. 60, no. 1, pp. 21–27, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. S. Yokota, Y. Onohara, and Y. Shoyama, “Immunofluorescence and immunoelectron microscopic localization of medicinal substance, Rb1, in several plant parts of Panax ginseng,” Current Drug Discovery Technologies, vol. 8, no. 1, pp. 51–59, 2011. View at Publisher · View at Google Scholar
  27. O. Morinaga, S. Zhu, H. Tanaka, and Y. Shoyama, “Visual detection of saikosaponins by on-membrane immunoassay and estimation of traditional Chinese medicines containing Bupleuri radix,” Biochemical and Biophysical Research Communications, vol. 346, no. 3, pp. 687–692, 2006. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  28. N. Fukuda, H. Tanaka, and Y. Shoyama, “Isolation of the pharmacologically active saponin ginsenoside Rb1 from ginseng by immunoaffinity column chromatography,” Journal of Natural Products, vol. 63, no. 2, pp. 283–285, 2000. View at Publisher · View at Google Scholar · View at Scopus
  29. H. Yanagihara, R. Sakata, H. Minami, H. Tanaka, Y. Shoyama, and H. Murakami, “Immunoaffinity column chromatography against forskolin using an anti-forskolin monoclonal antibody and its application,” Analytica Chimica Acta, vol. 335, no. 1-2, pp. 63–70, 1996. View at Publisher · View at Google Scholar · View at Scopus
  30. W. Putalun, H. Tanaka, and Y. Shoyama, “Rapid separation of solasodine glycosides by an immunoaffinity column using anti-solamargine monoclonal antibody,” Cytotechnology, vol. 31, no. 1-2, pp. 151–156, 1999. View at Scopus
  31. H. Tanaka, N. Fukuda, S. Yahara, S. Isoda, C. S. Yuan, and Y. Shoyama, “Isolation of ginsenoside Rb1 from Kalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody,” Phytotherapy Research, vol. 19, no. 3, pp. 255–258, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  32. S. Amagaya, E. Sugishita, and Y. Ogihara, “Comparative studies of the stereoisomers of glycyrrhetinic acid on anti-inflammatory activities,” Journal of Pharmacobio-Dynamics, vol. 7, no. 12, pp. 923–928, 1984.
  33. R. Doll and I.-D. Hill, “Triterpenoid liquorice compound in gastric and duodenal ulcer,” The Lancet, vol. 280, no. 7266, pp. 1166–1167, 1962. View at Scopus
  34. Z. Y. Wang and D. W. Nixon, “Licorice and cancer,” Nutrition and Cancer, vol. 39, no. 1, pp. 1–11, 2001. View at Scopus
  35. T. Kuroyanagi and M. Saito, “Effect of prednisolone and glycyrrhizin on passive transfer in experimental allergic encephalomyelitis,” Japanese Journal of Allergology, vol. 15, no. 2, pp. 67–74, 1966. View at Scopus
  36. T. Nakamura, T. Fujii, and A. Ichihara, “Enzyme leakage due to change of membrane permeability of primary cultured rat hepatocytes treated with various hepatotoxins and its prevention by glycyrrhizin,” Cell Biology and Toxicology, vol. 1, no. 4, pp. 285–295, 1985. View at Scopus
  37. M. N. Asl and H. Hosseinzadeh, “Review of pharmacological effects of glycyrrhiza sp. and its bioactive compounds,” Phytotherapy Research, vol. 22, no. 6, pp. 709–724, 2008. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  38. J. Xu, H. Tanaka, and Y. Shoyama, “One-step immunochromatographic separation and ELISA quantification of glycyrrhizin from traditional Chinese medicines,” Journal of Chromatography B, vol. 850, no. 1-2, pp. 53–58, 2007. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  39. C.-Z. Wang and Y. Shoyama, “Herbal medicine: identification, and evaluation strategies,” in Text Book of Complementary and Alternative Medicine, C.-S. Yuan, E.-J. Bieber, and B.-A. Bauer, Eds., pp. 51–70, Informa Healthcare, Minnesota, Minn, USA, 2nd edition, 2006.
  40. S. Moncada, R. M. J. Palmer, and E. A. Higgs, “Nitric oxide: physiology, pathophysiology, and pharmacology,” Pharmacological Reviews, vol. 43, no. 2, pp. 109–142, 1991. View at Scopus
  41. C. Nathan and Q. W. Xie, “Regulation of biosynthesis of nitric oxide,” Journal of Biological Chemistry, vol. 269, no. 19, pp. 13725–13728, 1994. View at Scopus
  42. K. M. Naseem, “The role of nitric oxide in cardiovascular diseases,” Molecular Aspects of Medicine, vol. 26, no. 1-2, pp. 33–65, 2005. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  43. T. J. Guzik, R. Korbut, and T. Adamek-Guzik, “Nitric oxide and superoxide in inflammation and immune regulation,” Journal of Physiology and Pharmacology, vol. 54, no. 4, pp. 469–487, 2003. View at Scopus
  44. S. B. Abramson, A. R. Amin, R. M. Clancy, and M. Attur, “The role of nitric oxide in tissue destruction,” Best Practice and Research: Clinical Rheumatology, vol. 15, no. 5, pp. 831–845, 2001. View at Publisher · View at Google Scholar · View at PubMed · View at Scopus
  45. T. Uto, O. Morinaga, H. Tanaka, and Y. Shoyama, “Analysis of the synergistic effect of glycyrrhizin and other constituents in licorice extract on lipopolysaccharide-induced nitric oxide production using knock-out extract,” Biochemical and Biophysical Research Communications, vol. 417, no. 1, pp. 473–478, 2012. View at Publisher · View at Google Scholar · View at PubMed
  46. K. Hashimoto, K. Satoh, P. Murata et al., “Components of Panax ginseng that improve accelerated small intestinal transit,” Journal of Ethnopharmacology, vol. 84, no. 1, pp. 115–119, 2003. View at Publisher · View at Google Scholar · View at Scopus