Low-Level Detection of Poly(amidoamine) PAMAM Dendrimers Using Immunoimaging Scanning Probe Microscopy

Figure 3

Calibration curves for (a) immunoimaging SPM assay and (b) HPLC analysis of G4 biotinylated PAMAM dendrimers. The SPM analysis represents the average number of dendrimers bound within a 1×1 μm area for different concentrations of dendrimers. Data acquired from 10 capture substrates, with 10 images per sample (𝑦=58.1𝑥+1.76, 𝑛=20, 𝑟²=0.9964). The HPLC analysis represents an average of 10 samples for each concentration evaluated (𝑦=34620.85𝑥+28845.984, 𝑛=10, 𝑟²=0.9986).

(a)

(b)