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International Journal of Analytical Chemistry
Volume 2012 (2012), Article ID 375831, 7 pages
http://dx.doi.org/10.1155/2012/375831
Research Article

Direct Electron Transfer of Hemoglobin on Manganese III Oxide-Ag Nanofibers Modified Glassy Carbon Electrode

1Department of Biology, Payam-e-Noor University, Tehran 7371719578, Iran
2Department of Biology, Shahrekord University, Shahrekord, Iran
3Department of Biology, Payam-e-Noor University, Taft, Iran
4Young Researchers Club, Islamic Azad University, Yazd Branch, Yazd, Iran

Received 30 December 2011; Accepted 4 February 2012

Academic Editor: Bahram Hemmateenejad

Copyright © 2012 Masoud Negahdary et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the electrochemical behavior of hemoglobin by glassy carbon electrode modified with Mn2O3-Ag nanofibers. The Mn2O3-Ag nanofibers were used as facilitator electron transfer between Hb and glassy-carbon-modified electrode. The Mn2O3-Ag nanofibers are studied by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The hemoglobin showed a quasireversible electrochemical redox behavior with a formal potential of −49 mV (versus Ag/AgCl) in 0.1 M potassium phosphate buffer solution at pH 7.0. The designed biosensor possesses good stability and reproducibility and achieves 95% of the steady-state current in less than five seconds.