Table 3: Parameters of , , , and between Avelox and BSA at different temperatures.

T/KE (%)J/(M−1 cm3) /nmr/nm

29328.041.07×10−142.482.90
30326.661.04×10−142.472.92
31325.601.02×10−142.462.95

is the critical distance when is 50%; r is the distance between acceptor and donor; J is the overlap integral between the fluorescence emission spectrum of donor and the absorption spectrum of the acceptor.