Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

Figure 2

Electropherogram of standard mixture in basic BGE. Peak identification: (1) B₁ 20 μg/mL; (2) B₃ 10 μg/mL; (3) B₂ 10 μg/mL; (4) B₆ 5 μg/mL; (5) B_1a 10 μg/mL; (6) B₁₀ 5 μg/mL; (7) B₅ + B_2a (40 + 10) μg/mL; (8) S.I. 3 μg/mL; (9) B₉ 20 μg/mL. Experimental conditions: borate buffer 20 mM; pH = 9.2; 𝑉 = 20 kV; 𝑖 = 25 μA; 𝑇 = 25°C; 𝑡_𝑖𝑛𝑗 = 5 s 𝑝_𝑖𝑛𝑗 = 0.5 psi fused silica capillary ID = 50 μm; 𝐿 = 59.5 cm; 𝑙 = 49.5 cm, instrument P/ACE MDQ System (Beckman).