Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations

<table>Electropherogram of standard mixture. Peak identification: (1) B<sub>1</sub>, (2) B<sub>3</sub>, (3) B<sub>2</sub>, (4) B<sub>6</sub>, (5) B<sub>1a</sub>, (6) B<sub>10</sub>, (7) B<sub>5</sub> + B<sub>2a</sub>, (8) B<sub>9.</sub> The vitamin concentrations are the same as in Figure <a href="../fig2/">2</a>. Experimental conditions: borate buffer 20 mM; pH = 9,2; <svg height="11.375" id="M46" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 12.7375 11.375" width="12.7375" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,11.113)"><path d="M730 650l-8 -28q-52 -4 -72 -18t-64 -77q-79 -113 -321 -539h-33l-119 541q-13 59 -29.5 73t-66.5 20l7 28h245l-8 -28l-28 -5q-33 -6 -40.5 -15.5t-0.5 -38.5l102 -450h2q191 320 246 430q21 42 15.5 56t-43.5 19l-31 4l7 28h240z" id="x1D449"></path></g>
</svg> = 10 kV; <svg height="11.4625" id="M47" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 4.7624998 11.4625" width="4.7624998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,11.2)"><path d="M244 607q0 -25 -15.5 -43t-37.5 -18q-19 0 -32 13t-13 35q0 21 15 41t39 20q20 0 32 -14t12 -34zM222 91q-29 -33 -79 -68t-75 -35q-13 0 -19 7.5t-6 31t10 65.5l62 253q5 26 -1 26q-21 0 -72 -43l-13 24q43 40 91 68t71 28q30 0 10 -78l-71 -274q-8 -30 3 -30
q16 0 76 48z" id="x1D456"></path></g>
</svg> = 17 <i>μ</i>A; <svg height="11.6125" id="M48" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.45 11.6125" width="11.45" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,11.55)"><path d="M649 676l-22 -187l-33 -2q3 56 -12 94q-8 20 -31.5 26.5t-86.5 6.5h-74l-90 -491q-4 -23 -6 -36.5t1 -25t7 -16.5t18 -9t27 -5t41 -3l-6 -28h-286l4 28q68 5 84 18.5t28 76.5l94 491h-55q-74 0 -100.5 -6t-41.5 -23q-24 -29 -54 -98l-32 1q32 98 53 188h22q7 -18 15 -22
t37 -4h417q23 0 33.5 5t25.5 21h23z" id="x1D447"></path></g>
</svg> = 25°C; hydrodynamic injection <svg height="17.375" id="M49" style="vertical-align:-5.76984pt" version="1.1" viewbox="0 0 20.799999 17.375" width="20.799999" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,10.125)"><path d="M324 430l-26 -36l-112 -4l-55 -265q-13 -66 7 -66q13 0 44.5 20t50.5 40l17 -24q-38 -40 -85.5 -73.5t-87.5 -33.5q-50 0 -21 138l55 262h-80l-2 8l25 34h66l25 99l78 63l10 -9l-37 -153h128z" id="x1D461"></path></g>
<g transform="matrix(.012,0,0,-.012,5.863,14.2)"><path d="M244 607q0 -25 -15.5 -43t-37.5 -18q-19 0 -32 13t-13 35q0 21 15 41t39 20q20 0 32 -14t12 -34zM222 91q-29 -33 -79 -68t-75 -35q-13 0 -19 7.5t-6 31t10 65.5l62 253q5 26 -1 26q-21 0 -72 -43l-13 24q43 40 91 68t71 28q30 0 10 -78l-71 -274q-8 -30 3 -30
q16 0 76 48z" id="x1D456"></path></g><g transform="matrix(.012,0,0,-.012,9.111,14.2)"><path d="M495 86q-46 -47 -87 -72.5t-63 -25.5q-43 0 -16 107l49 210q7 34 8 50.5t-3 21t-13 4.5q-35 0 -109.5 -72.5t-115.5 -140.5q-21 -75 -38 -159q-50 -10 -76 -21l-6 8l84 340q8 35 -4 35q-17 0 -67 -46l-15 26q44 44 85.5 70.5t64.5 26.5q35 0 10 -103l-24 -98h2
q42 56 97 103.5t96 71.5q46 26 74 26q9 0 16 -2.5t14 -11.5t9.5 -24.5t-1 -44t-13.5 -68.5q-30 -117 -47 -200q-4 -19 -3.5 -25t6.5 -6q21 0 70 48z" id="x1D45B"></path></g><g transform="matrix(.012,0,0,-.012,15.084,14.2)"><path d="M394 607q0 -25 -15.5 -43t-39.5 -18q-19 0 -32 13t-13 35t16 41.5t40 19.5q20 0 32 -13.5t12 -34.5zM360 349l-57 -283q-38 -188 -122 -263q-72 -64 -114 -64q-16 0 -30 10.5t-14 22.5q0 24 21 45q15 13 26 6q30 -20 54 -20q41 0 65 95q37 147 86 415q6 34 6.5 42.5
t-5.5 11.5q-18 0 -79 -54l-14 25q48 48 96.5 79t71.5 31q29 0 9 -99z" id="x1D457"></path></g>
</svg> = 1 s; fused silica capillary ID = 50 <i>μ</i>m; <svg height="11.175" id="M50" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 9.8249998 11.175" width="9.8249998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,11.113)"><path d="M559 163q-23 -66 -68 -163h-474l6 26q62 4 79.5 19.5t28.5 75.5l78 409q7 35 8.5 49t-8 25t-24 13t-51.5 5l5 28h266l-6 -28q-65 -5 -79.5 -18t-25.5 -74l-76 -406q-10 -57 14 -75q12 -13 96 -13q93 0 126 29q41 40 76 109z" id="x1D43F"></path></g>
</svg> = 44 cm; <svg height="12.4375" id="M51" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 4.6999998 12.4375" width="4.6999998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,0,0,-.017,.062,12.163)"><path d="M238 681l-124 -585q-7 -31 4 -31q10 0 37.5 18.5t49.5 41.5l16 -22q-40 -48 -89.5 -81.5t-76.5 -33.5q-42 0 -16 122l105 488q7 32 0 41t-39 9h-35l5 26q35 3 71 13t58 17.5t26 7.5q14 0 8 -31z" id="x1D459"></path></g>
</svg> = 36 cm; instrument Spectra Phoresis 1000.</table>

International Journal of Analytical Chemistry

fig4

Figure 4

Figure 4: Application of CZE Method in Routine Analysis for Determination of B-Complex Vitamins in Pharmaceutical and Veterinary Preparations 