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International Journal of Analytical Chemistry
Volume 2012 (2012), Article ID 809513, 7 pages
http://dx.doi.org/10.1155/2012/809513
Research Article

Micellar Liquid Chromatographic Determination of Carbaryl and 1-Naphthol in Water, Soil, and Vegetables

1Grup de Química Bioanalítica, Q.F.A., E.S.T.C.E., Universitat Jaume I, 12071 Castelló, Spain
2Pfizer Analytical Research Centre, Ghent University, B-9000 Ghent, Belgium
3Department of Applied Chemistry, IT, Banaras Hindu University, 221005 Varanasi, India
4Department of Criminology and Forensic Sciences, Dr. H. S. Gour University, 470003 Sagar, India

Received 28 September 2011; Accepted 27 October 2011

Academic Editor: Dimitrios P. Nikolelis

Copyright © 2012 Mei-Liang Chin-Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A liquid chromatographic procedure has been developed for the determination of carbaryl, a phenyl-N-methylcarbamate, and its main metabolite 1-naphthol, using a C18 column (250 mm  ×  4.6 mm) with a micellar mobile phase and fluorescence detection at maximum excitation/emission wavelengths of 225/333 nm, respectively. In the optimization step, surfactants sodium dodecyl sulphate (SDS), Brij-35 and N-cetylpyridinium chloride monohydrate, and organic solvents propanol, butanol, and pentanol were considered. The selected mobile phase was 0.15 M SDS-6% (v/v)-pentanol-0.01 M NaH2PO4 buffered at pH 3. Validation studies, according to the ICH Tripartite Guideline, included linearity ( 𝑟 > 0 . 9 9 9 ), limit of detection (5 and 18 ng mL−1, for carbaryl and 1-naphthol, resp.), and limit of quantification (15 and 50 ng mL−1, for carbaryl and 1-naphthol, resp.), with intra- and interday precisions below 1%, and robustness parameters below 3%. The results show that the procedure was adequate for the routine analysis of these two compounds in water, soil, and vegetables samples.