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International Journal of Analytical Chemistry
Volume 2012 (2012), Article ID 850969, 7 pages
http://dx.doi.org/10.1155/2012/850969
Research Article

Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine

1Instituto Federal de Educação, Ciência e Tecnologia do Piauí (IFPI), Campus Parnaíba, 64210260 Parnaíba, PI, Brazil
2Biotec, Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Universidade Federal do Piauí (UFPI), Campus Ministro Reis Velloso (CMRV), 64202020 Parnaíba, PI, Brazil
3Departamento de Química, Centro de Ciências da Natureza (CCN), Universidade Federal do Piauí (UFPI), 64049550 Teresina, PI, Brazil
4Grupo de Biofísica Molecular Sérgio Mascarenhas, Instituto de Física de São Carlos (IFSC), USP, 13560970 São Carlos, SP, Brazil

Received 5 September 2011; Accepted 3 October 2011

Academic Editor: Ricardo Vessecchi

Copyright © 2012 Maysa F. Zampa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries.