Liquid Chromatography/Tandem Mass Spectrometry Method for Quantitation of Cremophor EL and Its Applications

Table 1

Calculated concentrations and statistical parameters of CrEL-PEG calibration standards prepared in rat plasma (𝑛=3).

Concentration (µg/mL)				Statistical parameters
Actual conc.	Calculated conc.			Mean	SD	CV%	Relative error (%)
	Set 1	Set 2	Set 3
1.00	1.07	1.04	1.05	1.05	0.02	1.45	5.33
1.50	1.40	1.41	1.41	1.41	0.01	0.41	−6.22
5.00	4.56	5.15	4.95	4.89	0.30	6.14	−2.27
20.00	18.84	19.05	18.54	18.81	0.26	1.36	−5.95
70.00	70.19	67.98	68.39	68.85	1.18	1.71	−1.64
120.00	122.31	122.36	127.58	124.08	3.03	2.44	3.40
160.00	173.41	176.10	152.36	167.29	13.00	7.77	4.56
180.00	187.32	174.58	194.31	185.40	10.00	5.40	3.00
200.00	199.88	196.18	205.05	200.37	4.46	2.22	0.19