Two Validated Spectrofluorometric Methods for Determination of Gemifloxacin Mesylate in Tablets and Human Plasma

Table 1

Analytical parameters for the determination of GFX using the two proposed methods.

Parameter	Micelle-enhanced spectrofluorometric method	Hantzsch reaction method
Range (ng/mL)	10–1000	100–2000
Intercept (𝑎) ±SD^a	−0.50 ± 0.10	1.28 ± 0.07
Slope (𝑏) ± SD^a	0.13±2×10^−4	0.01±4×10^−3
Correlation coefficient (r)	0.9999	0.9988
LOD (ng/mL)	2.32	20.45
LOQ (ng/mL)	7.74	68.18

aMean of six determinations.