Comparative Analysis of Amino Acids, Nucleosides, and Nucleobases in Thais clavigera from Different Distribution Regions by Using Hydrophilic Interaction Ultra-Performance Liquid Chromatography Coupled with Triple Quadrupole Tandem Mass Spectrometry
Table 2
Contents (mg/g) of amino acids, nucleosides, and nucleobases in Thais clavigera.
Analytes
Sample (mg/g, )
1
2
3
4
5
6
7
8
9
10
Adenosine--monophosphate
0.17 ± 0.02
0.18 ± 0.02
0.20 ± 0.02
0.18 ± 0.03
0.18 ± 0.03
0.21 ± 0.05
0.16 ± 0.02
0.15 ± 0.03
0.15 ± 0.00
0.15 ± 0.03
Inosine
1.80 ± 0.29
0.44 ± 0.05
1.55 ± 0.06
1.92 ± 0.06
1.00 ± 0.19
0.41 ± 0.03
2.54 ± 0.31
1.30 ± 0.16
0.93 ± 0.14
0.31 ± 0.03
Guanosine
0.09 ± 0.01
0.09 ± 0.01
0.17 ± 0.02
0.08 ± 0.02
0.33 ± 0.07
0.13 ± 0.07
0.10 ± 0.02
0.33 ± 0.04
0.15 ± 0.02
0.05 ± 0.02
Thymidine
0.85 ± 0.02
0.98 ± 0.10
0.92 ± 0.09
0.73 ± 0.06
0.63 ± 0.13
1.11 ± 0.31
0.18 ± 0.02
2.00 ± 0.21
+b
0.15 ± 0.02
2′-Deoxyuridine
0.22 ± 0.02
0.53 ± 0.04
0.15 ± 0.02
0.16 ± 0.02
0.18 ± 0.01
0.45 ± 0.05
+
0.15 ± 0.03
0.16 ± 0.03
0.70 ± 0.17
2′-Deoxyinosine
0.53 ± 0.06
0.16 ± 0.02
0.64 ± 0.08
0.41 ± 0.05
0.44 ± 0.06
0.22 ± 0.04
0.40 ± 0.08
0.46 ± 0.06
0.35 ± 0.05
0.61 ± 0.07
Cytidine-5′-monophosphate
0.19 ± 0.05
0.16 ± 0.01
0.20 ± 0.04
0.16 ± 0.02
0.17 ± 0.03
0.19 ± 0.04
0.17 ± 0.04
0.17 ± 0.02
0.16 ± 0.01
0.18 ± 0.02
2′-Deoxyadenosine-5′-monophosphate
0.16 ± 0.02
0.27 ± 0.02
0.17 ± 0.02
0.24 ± 0.09
0.17 ± 0.04
0.26 ± 0.03
0.12 ± 0.01
0.17 ± 0.02
0.12 ± 0.00
0.15 ± 0.04
2′-Deoxycytidine
0.36 ± 0.05
0.17 ± 0.02
0.56 ± 0.06
0.43 ± 0.02
0.43 ± 0.03
0.28 ± 0.05
0.39 ± 0.06
0.44 ± 0.00
0.28 ± 0.01
0.15 ± 0.00
2′-Deoxyguanosine
1.04 ± 0.27
0.58 ± 0.16
1.81 ± 0.09
0.66 ± 0.16
0.76 ± 0.05
0.77 ± 0.03
0.59 ± 0.22
0.81 ± 0.02
0.12 ± 0.12
0.21 ± 0.14
Thymine
0.73 ± 0.05
0.36 ± 0.09
0.72 ± 0.06
1.88 ± 0.21
1.75 ± 0.20
0.40 ± 0.03
0.85 ± 0.08
0.82 ± 0.07
+
0.30 ± 0.05
Adenine
0.26 ± 0.03
0.28 ± 0.07
0.18 ± 0.02
0.14 ± 0.07
0.12 ± 0.01
0.24 ± 0.01
0.22 ± 0.11
0.22 ± 0.04
+
0.19 ± 0.03
Cytidine
0.07 ± 0.03
0.09 ± 0.01
0.08 ± 0.01
0.07 ± 0.02
0.55 ± 0.05
0.85 ± 0.04
+
0.52 ± 0.00
+
+
Uracil
+
0.74 ± 0.06
+
0.35 ± 0.04
+
0.81 ± 0.03
0.51 ± 0.05
0.45 ± 0.02
+
0.63 ± 0.03
Guanine
0.11 ± 0.02
0.95 ± 0.05
0.11 ± 0.04
0.13 ± 0.06
0.15 ± 0.01
0.96 ± 0.02
0.11 ± 0.01
0.14 ± 0.00
nd
+
Xanthine
0.20 ± 0.06
0.21 ± 0.01
0.18 ± 0.03
0.20 ± 0.04
0.23 ± 0.09
0.21 ± 0.04
0.13 ± 0.05
0.17 ± 0.00
0.14 ± 0.03
0.22 ± 0.02
Glycine
21.27 ± 2.47
13.21 ± 1.87
16.13 ± 2.42
18.07 ± 2.04
12.79 ± 1.54
12.93 ± 0.99
20.65 ± 0.78
15.25 ± 1.03
13.43 ± 1.02
25.00 ± 2.06
GABA
0.17 ± 0.02
0.15 ± 0.02
0.08 ± 0.00
0.06 ± 0.02
0.06 ± 0.01
0.09 ± 0.01
0.09 ± 0.00
0.12 ± 0.04
0.08 ± 0.01
0.06 ± 0.02
Leucine
6.11 ± 0.59
5.64 ± 1.21
7.64 ± 0.57
5.28 ± 0.31
7.78 ± 1.02
7.36 ± 1.18
5.94 ± 0.67
5.39 ± 1.02
5.03 ± 1.49
5.43 ± 0.37
Isoleucine
3.82 ± 0.34
3.72 ± 0.09
4.47 ± 1.16
2.96 ± 1.06
5.17 ± 1.08
4.52 ± 0.16
2.79 ± 0.07
5.05 ± 0.18
6.27 ± 1.27
8.54 ± 1.14
Methionine
1.37 ± 0.16
2.74 ± 0.86
1.45 ± 0.31
2.72 ± 0.55
1.04 ± 0.12
0.96 ± 0.22
2.73 ± 0.22
1.07 ± 0.26
1.70 ± 0.21
1.82 ± 0.32
Phenylalanine
4.72 ± 0.24
7.19 ± 0.53
5.49 ± 0.38
5.43 ± 1.27
8.78 ± 0.72
10.29 ± 1.03
5.78 ± 1.47
8.58 ± 0.43
5.22 ± 0.73
6.19 ± 1.15
Tryptophan
0.95 ± 0.06
1.96 ± 0.84
1.07 ± 0.24
1.10 ± 0.39
1.72 ± 0.56
2.18 ± 0.58
2.16 ± 1.32
3.24 ± 1.39
1.86 ± 0.57
3.20 ± 1.64
Alanine
11.97 ± 2.37
12.64 ± 1.81
12.92 ± 1.65
14.18 ± 0.83
18.06 ± 2.59
15.18 ± 1.42
15.46 ± 1.01
19.52 ± 0.43
12.21 ± 1.04
13.06 ± 0.27
Threonine
6.15 ± 0.27
6.73 ± 0.70
5.42 ± 0.64
5.82 ± 0.52
5.52 ± 0.83
6.34 ± 1.01
4.98 ± 0.45
5.65 ± 1.63
4.31 ± 0.43
6.16 ± 0.50
Serine
3.98 ± 0.67
4.54 ± 0.52
5.44 ± 0.29
4.03 ± 1.73
5.37 ± 0.37
6.18 ± 0.82
4.11 ± 1.09
5.33 ± 0.45
4.21 ± 0.62
5.94 ± 0.14
Asparagine
1.60 ± 0.18
1.85 ± 1.44
2.13 ± 1.04
1.76 ± 0.53
2.11 ± 0.67
2.49 ± 0.32
1.06 ± 0.11
1.10 ± 0.17
1.66 ± 0.24
1.67 ± 0.42
Glutamine
2.56 ± 0.38
3.07 ± 0.14
3.79 ± 0.23
2.32 ± 0.82
4.24 ± 0.36
4.57 ± 0.86
2.24 ± 1.08
3.58 ± 1.49
2.08 ± 1.14
2.25 ± 0.52
Glutamate
9.52 ± 0.50
15.87 ± 0.71
10.62 ± 0.38
15.54 ± 0.98
17.46 ± 1.28
19.63 ± 0.46
14.76 ± 1.05
17.67 ± 1.39
8.54 ± 0.27
9.94 ± 1.02
Citrulline
0.67 ± 0.11
0.72 ± 0.00
0.54 ± 0.01
0.62 ± 0.02
0.62 ± 0.01
0.52 ± 0.04
0.52 ± 0.02
0.56 ± 0.01
0.57 ± 0.00
0.48 ± 0.01
Proline
2.87 ± 0.12
3.14 ± 0.14
2.78 ± 0.11
3.15 ± 0.12
3.84 ± 0.09
3.28 ± 0.26
3.10 ± 0.15
4.06 ± 0.26
2.44 ± 0.19
2.88 ± 0.07
Hydroxyproline
0.50 ± 0.06
0.46 ± 0.00
0.51 ± 0.04
0.48 ± 0.02
0.48 ± 0.03
0.52 ± 0.05
0.50 ± 0.02
0.49 ± 0.01
0.24 ± 0.01
0.25 ± 0.00
Cysteine
+
+
+
+
+
+
+
+
+
+
Tyrosine
2.17 ± 0.19
3.88 ± 0.07
3.03 ± 0.08
6.92 ± 0.15
9.28 ± 0.24
5.96 ± 1.08
5.37 ± 1.80
7.29 ± 0.51
4.03 ± 1.22
4.71 ± 1.01
Taurine
14.85 ± 1.86
17.62 ± 1.34
13.25 ± 1.70
5.98 ± 0.39
8.30 ± 0.34
18.73 ± 0.24
17.36 ± 1.66
9.56 ± 1.10
10.72 ± 0.57
8.05 ± 0.39
Valine
4.65 ± 0.77
6.45 ± 0.23
5.12 ± 1.08
6.48 ± 0.51
7.86 ± 0.17
7.45 ± 1.02
6.51 ± 0.24
7.83 ± 1.14
7.66 ± 2.09
9.18 ± 1.44
Ornithine
3.91 ± 0.41
3.95 ± 0.87
3.44 ± 0.62
4.50 ± 0.19
6.13 ± 1.25
3.90 ± 1.09
4.74 ± 0.37
4.43 ± 0.76
4.35 ± 1.05
5.29 ± 0.52
Aspartate
3.85 ± 0.13
4.23 ± 0.15
5.13 ± 1.29
4.16 ± 0.78
5.48 ± 0.52
6.17 ± 1.32
6.10 ± 1.22
11.05 ± 0.56
5.79 ± 1.33
8.96 ± 1.01
Lysine
15.58 ± 1.98
14.78 ± 1.39
16.05 ± 1.14
15.72 ± 0.38
19.10 ± 1.58
17.50 ± 0.49
17.10 ± 1.28
19.80 ± 1.72
11.85 ± 0.85
13.12 ± 2.45
Histidine
3.04 ± 0.54
3.36 ± 0.82
3.79 ± 0.73
2.80 ± 0.66
3.52 ± 1.19
4.36 ± 0.44
4.14 ± 0.19
5.31 ± 0.21
2.21 ± 0.31
2.81 ± 0.56
Arginine
3.35 ± 0.23
3.75 ± 1.04
3.05 ± 1.09
4.16 ± 0.84
2.94 ± 0.62
4.13 ± 1.67
4.01 ± 0.89
3.12 ± 0.25
7.35 ± 0.87
8.56 ± 0.38
Nd
7.06 ± 0.85
6.20 ± 0.60
7.92 ± 0.67
7.75 ± 0.83
7.33 ± 0.86
7.53 ± 0.73
6.65 ± 0.94
6.51 ± 0.58
3.21 ± 0.27
4.01 ± 0.53
Ae
129.83 ± 13.20
141.77 ± 15.34
133.55 ± 14.75
144.39 ± 13.66
157.79 ± 15.74
165.37 ± 15.31
152.30 ± 15.71
167.12 ± 15.00
123.92 ± 16.08
153.70 ± 15.96
Total
136.90 ± 14.04
147.98 ± 15.94
141.47 ± 15.41
152.14 ± 14.49
165.12 ± 16.60
172.90 ± 16.04
158.95 ± 16.65
173.64 ± 15.57
127.13 ± 16.35
157.72 ± 16.49
Analytes
Sample (mg/g, )
11
12
13
14
15
16
17
18
19
Adenosine--monophosphate
0.21 ± 0.02
0.18 ± 0.02
0.18 ± 0.02
0.18 ± 0.01
0.16 ± 0.01
0.15 ± 0.01
0.81 ± 0.08
0.76 ± 0.07
0.82 ± 0.03
Inosine
0.57 ± 0.06
0.75 ± 0.08
0.67 ± 0.10
0.42 ± 0.03
1.60 ± 0.21
0.84 ± 0.11
3.61 ± 0.34
2.15 ± 0.23
2.21 ± 0.19
Guanosine
0.06 ± 0.02
0.05 ± 0.01
0.09 ± 0.02
0.06 ± 0.00
0.15 ± 0.01
0.10 ± 0.02
0.84 ± 0.01
0.40 ± 0.03
0.48 ± 0.05
Thymidine
ndc
nd
+
0.18 ± 0.00
+
0.14 ± 0.01
0.57 ± 0.01
0.64 ± 0.11
0.43 ± 0.08
2′-Deoxyuridine
+
+
0.38 ± 0.02
0.75 ± 0.02
0.19 ± 0.01
0.61 ± 0.07
0.61 ± 0.07
0.60 ± 0.12
0.61 ± 0.08
2′-Deoxyinosine
0.21 ± 0.02
0.24 ± 0.03
0.42 ± 0.06
0.90 ± 0.09
0.69 ± 0.08
1.14 ± 0.03
2.67 ± 0.34
2.86 ± 1.01
1.92 ± 0.91
Cytidine-5′-monophosphate
+
+
0.19 ± 0.03
0.18 ± 0.02
0.17 ± 0.01
0.18 ± 0.01
0.32 ± 0.01
0.27 ± 0.00
0.26 ± 0.02
2′-Deoxyadenosine-5′-monophosphate
0.15 ± 0.01
0.16 ± 0.05
0.13 ± 0.02
0.16 ± 0.02
0.21 ± 0.00
0.13 ± 0.01
0.35 ± 0.03
0.37 ± 0.04
0.23 ± 0.01
2′-Deoxycytidine
0.21 ± 0.02
0.15 ± 0.00
0.21 ± 0.03
0.16 ± 0.01
0.22 ± 0.02
0.22 ± 0.04
0.34 ± 0.00
0.41 ± 0.01
0.73 ± 0.04
2′-Deoxyguanosine
0.13 ± 0.03
0.11 ± 0.01
0.23 ± 0.01
0.50 ± 0.01
0.12 ± 0.01
0.21 ± 0.00
3.15 ± 0.19
2.87 ± 0.22
2.25 ± 0.54
Thymine
+
+
+
0.43 ± 0.02
+
0.35 ± 0.02
1.57 ± 0.21
1.76 ± 0.12
1.24 ± 0.42
Adenine
+
+
0.12 ± 0.00
0.14 ± 0.01
+
0.18 ± 0.01
0.85 ± 0.03
0.67 ± 0.02
0.57 ± 0.02
Cytidine
+
+
0.12 ± 0.02
0.09 ± 0.00
0.07 ± 0.00
+
0.08 ± 0.01
0.20 ± 0.02
0.21 ± 0.02
Uracil
0.40 ± 0.12
0.45 ± 0.02
0.75 ± 0.03
1.24 ± 0.22
0.46 ± 0.02
1.48 ± 0.53
2.51 ± 0.66
2.81 ± 0.39
2.47 ± 0.32
Guanine
nd
nd
+
+
+
+
0.41 ± 0.02
0.32 ± 0.02
0.27 ± 0.03
Xanthine
0.13 ± 0.04
+
0.17 ± 0.01
0.23 ± 0.02
0.14 ± 0.01
0.22 ± 0.01
1.80 ± 0.07
1.44 ± 0.05
1.44 ± 0.05
Glycine
11.86 ± 2.16
10.73 ± 1.06
15.31 ± 1.22
22.54 ± 3.53
11.79 ± 0.54
22.36 ± 2.02
30.29 ± 3.09
31.58 ± 2.56
30.06 ± 3.60
GABA
0.10 ± 0.01
0.09 ± 0.00
0.20 ± 0.01
0.20 ± 0.02
0.13 ± 0.01
0.21 ± 0.01
2.47 ± 0.14
2.08 ± 0.17
1.20 ± 0.10
Leucine
6.65 ± 0.78
5.48 ± 0.96
4.96 ± 1.86
5.70 ± 1.94
6.19 ± 1.65
6.25 ± 0.97
10.43 ± 2.34
10.58 ± 2.25
8.74 ± 1.16
Isoleucine
5.12 ± 0.57
5.99 ± 1.22
7.00 ± 1.67
8.38 ± 1.41
3.51 ± 1.38
5.07 ± 0.70
8.78 ± 1.80
9.12 ± 0.84
8.90 ± 2.59
Methionine
1.72 ± 0.02
1.48 ± 0.16
2.44 ± 0.57
2.64 ± 0.18
2.73 ± 0.07
2.97 ± 0.84
7.64 ± 0.65
8.44 ± 0.16
5.82 ± 1.97
Phenylalanine
8.24 ± 1.28
7.35 ± 1.31
11.21 ± 0.54
12.94 ± 1.60
12.33 ± 1.52
16.13 ± 2.01
16.87 ± 1.49
17.44 ± 2.60
17.58 ± 1.99
Tryptophan
1.20 ± 0.25
0.96 ± 0.16
1.14 ± 0.24
1.50 ± 0.19
1.11 ± 0.25
1.98 ± 0.49
3.31 ± 0.55
3.06 ± 0.07
3.03 ± 0.97
Alanine
9.26 ± 0.49
8.82 ± 2.35
14.90 ± 0.26
12.03 ± 0.62
11.78 ± 0.59
13.28 ± 1.80
27.24 ± 2.16
27.08 ± 1.02
27.44 ± 2.00
Threonine
3.85 ± 2.16
2.98 ± 0.32
4.71 ± 0.08
5.72 ± 0.14
4.55 ± 0.21
5.76 ± 0.23
17.74 ± 1.58
18.72 ± 2.06
16.63 ± 1.07
Serine
3.50 ± 0.23
3.12 ± 0.12
4.94 ± 0.13
5.25 ± 0.32
4.22 ± 0.11
6.14 ± 0.92
13.60 ± 2.13
16.12 ± 1.14
13.30 ± 2.10
Asparagine
0.99 ± 1.06
1.04 ± 0.85
3.12 ± 2.11
3.42 ± 0.21
1.69 ± 0.48
1.72 ± 0.21
3.72 ± 0.57
4.33 ± 0.28
2.71 ± 0.34
Glutamine
2.07 ± 0.79
2.57 ± 1.42
4.52 ± 0.83
5.42 ± 1.51
4.99 ± 0.60
5.50 ± 0.18
8.46 ± 1.13
10.08 ± 0.72
6.49 ± 1.04
Glutamate
8.42 ± 0.62
9.28 ± 0.16
8.91 ± 1.01
9.97 ± 0.86
8.19 ± 1.94
10.75 ± 1.65
22.86 ± 0.97
23.74 ± 2.11
21.73 ± 1.84
Citrulline
0.35 ± 0.81
0.25 ± 0.02
1.07 ± 0.04
0.99 ± 0.05
0.94 ± 0.04
0.97 ± 0.03
2.09 ± 0.09
1.56 ± 0.04
1.56 ± 0.11
Proline
2.68 ± 0.13
2.93 ± 0.77
5.74 ± 0.67
5.52 ± 0.95
4.73 ± 0.18
5.65 ± 1.03
6.98 ± 0.74
6.73 ± 0.21
5.48 ± 0.93
Hydroxyproline
0.22 ± 0.01
0.21 ± 0.01
0.50 ± 0.02
0.54 ± 0.03
0.49 ± 0.02
0.52 ± 0.00
4.28 ± 0.50
4.37 ± 0.10
3.92 ± 0.33
Cysteine
nd
nd
+
+
+
+
0.35 ± 0.00
0.35 ± 0.01
0.31 ± 0.01
Tyrosine
5.32 ± 0.20
5.83 ± 0.05
4.27 ± 0.09
4.63 ± 0.14
9.49 ± 1.17
11.81 ± 0.96
7.36 ± 0.23
7.66 ± 0.38
7.53 ± 0.09
Taurine
11.90 ± 0.76
11.08 ± 1.23
15.59 ± 1.18
14.89 ± 1.02
10.06 ± 0.92
7.24 ± 0.14
19.61 ± 1.35
20.78 ± 2.11
17.85 ± 1.39
Valine
5.29 ± 0.45
6.02 ± 0.18
7.47 ± 0.79
8.59 ± 0.29
4.33 ± 0.29
5.53 ± 0.15
9.55 ± 0.51
9.64 ± 0.64
8.22 ± 1.01
Ornithine
6.67 ± 0.50
6.64 ± 0.37
10.01 ± 0.57
3.81 ± 0.09
6.15 ± 0.84
5.62 ± 0.84
5.98 ± 0.38
5.82 ± 0.22
6.67 ± 0.76
Aspartate
8.09 ± 0.29
7.63 ± 0.79
8.03 ± 0.44
8.88 ± 1.00
5.23 ± 1.91
9.18 ± 0.62
7.73 ± 0.33
8.53 ± 0.58
6.97 ± 0.25
Lysine
8.19 ± 0.63
8.55 ± 0.54
12.65 ± 0.82
14.52 ± 1.25
16.40 ± 1.09
16.26 ± 1.13
15.07 ± 0.86
15.72 ± 0.81
15.45 ± 0.65
Histidine
2.07 ± 0.61
1.85 ± 0.26
2.86 ± 0.09
2.98 ± 0.05
2.23 ± 0.30
2.85 ± 0.02
11.93 ± 0.32
12.00 ± 0.58
11.37 ± 0.29
Arginine
5.28 ± 0.18
5.82 ± 0.37
6.98 ± 0.66
8.52 ± 0.38
15.14 ± 0.54
15.94 ± 1.07
18.76 ± 1.39
20.12 ± 2.11
20.99 ± 1.27
Nd
2.65 ± 0.20
2.68 ± 0.08
4.04 ± 0.23
5.70 ± 0.51
4.64 ± 0.25
6.05 ± 0.74
20.49 ± 1.94
18.52 ± 2.32
16.15 ± 2.67
Ae
119.11 ± 14.00
116.74 ± 13.78
158.64 ± 14.45
170.61 ± 16.33
146.74 ± 15.20
179.52 ± 16.56
289.02 ± 23.85
298.58 ± 22.32
269.38 ± 26.41
Total
121.75 ± 14.19
119.41 ± 13.86
162.69 ± 14.68
176.31 ± 16.84
151.37 ± 15.45
185.57 ± 17.30
309.51 ± 25.79
317.10 ± 24.64
285.53 ± 29.08
The data was presented as average of three batches of samples. aSample number. bBelow the limit of quantitation. cUndetected. dTotal content of nucleosides and nucleobases. eTotal content of amino acids.
