Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth.

Figure 3

HPLC chromatogram of 80% ethanol extract of D. heterophyllum. Mobile phase: methanol, 0.1% HCOOH in water; flow rate: 1 mL/min; detection: 254 nm. 1: Luteolin, 2: kaempferol, 3: diosmetin, and 4: chrysosplenetin.