Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth.

Table 1

Results of the quantification of flavonoids in Dracocephalum  heterophyllum Benth.

Flavonoid	Regression equation	value	Percentage	SD	RSD	LOD (%)	LOQ (%)
Luteolin		0.9998	0.08	0.01	0.15	0.04	0.08
Kaempferol		0.9999	0.14	0.01	0.08	0.03	0.12
Diosmetin		0.9999	0.28	0.01	0.04	0.03	0.11
Chrysosplenetin		0.9998	0.79	0.03	0.04	0.08	0.28

SD: standard deviation; RSD: relative standard deviation; LOD: limit of detection; LOQ: limit of quantification; percentage of the dried extract.