Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Figure 4

Intracellular concentrations of FLC for two different C. albicans strains long-term cultivated in the presence or absence of FLC. All data represent mean ± SD (error bars) for , for each fungal culture, and are significantly different comparing initial Candida albicans NCTC-885-653 and Candida albicans ATCC-10231 strains at and comparing 20th generation of Candida albicans NCTC-885-653 and Candida albicans ATCC-10231 strains at , respectively.