Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Table 1

Intraday and interday precision and accuracy data for FLC assay in C. albicans matrix.

Concentration of analyte added (ng/mL)	Concentration of analyte found (ng/mL)	Intraday; (RSD, %)	Interday; (RSD, %)	Relative error (RE, %)
250	252 ± 7	2.79	2.64	0.68
2500	2571 ± 61	2.35	2.99	2.82
8000	8038 ± 59	0.73	1.05	0.47

Mean and SD representation for standard samples for each of the mentioned analytes. RSD, % = 100 × (SD/mean). RE, % = () × (100/), where is the calculated concentration and is the introduced concentration of the analyte.