Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Table 3

MIC values (μg/mL) for antifungal drugs FLC, VRC, and AmB of C. albicans initial andazole-resistantstrains, cultivated for 20 generations in the presence of FLC.

Fungal strain/antifungal drug	C. albicans; NCTC-885-653	C. albicans ATCC-10231
Initial strain
FLC	500	250
VRC	62.5	15.6
AmB	0.25	0.25
20th generation of FLC-resistant strain
FLC	500	750
VRC	250	500
AmB	0.125	0.500

The MIC was considered to be the concentration of drug that inhibited 100% of fungal growth. The fungal strains were propagated in the presence or absence of 1/25 of MIC concentration of FLC for a total of 20 generations and MIC value for FLC and VRC of each strain.