Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance

Table 4

MIC values for FLC and FLC intracellular concentration (μg/mL) of C. albicans azole-resistant strains and five different C. albicans clinical isolates.

Fungal strains	MIC for FLC	FLC intracellular concentration
C. albicans clinical isolates
Number 1	7.81	13 ± 1
Number 2	31.25	4 ± 0.2
Number 3	31.25	4 ± 0.2
Number 4	62.50	10 ± 1
Number 5	62.50	7 ± 0.4
C. albicans azole-resistant strains
NCTC-885-653	500	0.6 ± 0.01
ATCC-10231	750	0.3 ± 0.02

C. albicans azole-resistant NCTC-885-653 and ATCC-10231 strains at 20th generation and five different C. albicans clinical isolates strains were propagated in the presence of 20 g/mL concentration of FLC for a total of 30 min and FLC intracellular concentration was determined for each strain. The MIC was considered to be the concentration of FLC that inhibited 100% of fungal growth during 48 h incubation time. All data represent mean ± SD for , for each fungal culture, and are significantly different comparing C. albicans NCTC-885-653 and ATCC-10231 strains with clinical isolate at and comparing C. albicans clinical isolate number 1 with numbers 2–5 at and clinical isolates number 2 and number 3 with isolates number 4 and number 5 at , respectively.