Research Article
Development and Validation of Simple RP-HPLC Method for Intracellular Determination of Fluconazole Concentration and Its Application to the Study of Candida albicans Azole Resistance
Table 4
MIC values for FLC and FLC intracellular concentration (μg/mL) of C. albicans azole-resistant strains and five different C. albicans clinical isolates.
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C. albicans azole-resistant NCTC-885-653 and ATCC-10231 strains at 20th generation and five different C. albicans clinical isolates strains were propagated in the presence of 20 g/mL concentration of FLC for a total of 30 min and FLC intracellular concentration was determined for each strain. The MIC was considered to be the concentration of FLC that inhibited 100% of fungal growth during 48 h incubation time. All data represent mean ± SD for , for each fungal culture, and are significantly different comparing C. albicans NCTC-885-653 and ATCC-10231 strains with clinical isolate at and comparing C. albicans clinical isolate number 1 with numbers 2–5 at and clinical isolates number 2 and number 3 with isolates number 4 and number 5 at , respectively. |