Research Article
Dramatic Improvement of Proteomic Analysis of Zebrafish Liver Tumor by Effective Protein Extraction with Sodium Deoxycholate and Heat Denaturation
Table 1
Overview of the various lysis buffer and the number of proteins identified using 1D LC-MS/MS shotgun analysis.
| Number | Lysis buffer | Buffer component | Heat | Number of protein ID |
| 1 | SDS | 1% SDS, 0.5 M TEAB | − | NA. | 2 | SDS | 1% SDS, 0.5 M TEAB | + | 622 | 3 | RIPA | 50 mM Tris, 150 mM NaCl, 1% NP-40, 1% DOC, and 0.1% SDS | − | NA. | 4 | Urea | 9 M urea | − | NA. | 5 | 2D | 7 M urea, 2 M thiourea, 4% CHAPS, 20 mM DTT, and 40 mM Tris | − | NA. | 6 | DOC | 5% DOC | − | NA. | 7 | DOC | 5% DOC | + | 823 | 8 | TUTS | 25 mM TEAB, 8 M urea, 2% triton X-100, and 0.1% SDS | − | NA. |
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TEAB: triethylammonium bicarbonate; TUTS: TEAB/urea/triton-X/SDS.
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