Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

Table 3

ANOVA results for optimization by BBD.

Effect	SS	D.f.	MS	-ratio	value
	239422	1	239422	1.95	0.2216
	189543	1	189543	1.54	0.2694
	9489050	1	9489050	77.20	0.0003
	86144	1	86144	0.70	0.4407
	0	1	0	0.00	1.0000
	478304	1	478304	3.89	0.1056
	86239	1	86239	0.70	0.4404
	378422	1	378422	3.08	0.1397
	4374670	1	4374670	35.59	0.0019
Total error	614539	5	122908
Total (corr.)	15999393	14

SS: sum of squares; MS: mean squares; -ratio: MS/M; value: probability level; D.f.: degree of freedom.
, (adjusted for D.f.) = 0.892.
Significant factor at α = 0.05.