Development and Validation of HPLC Method for the Simultaneous Determination of Five Food Additives and Caffeine in Soft Drinks

Table 4

The important parameters of the calibration equations for the proposed HPLC method for simultaneous determination of potassium sorbate (SOR), sodium benzoate (BEN), carmoisine (CAR), allura red (ALU), ponceau 4R (PON), and caffeine (CAF).

Compounds	Calibration range; (μg/mL)	Regression equation; ( = + )				LOD; (μg/mL)	LOQ; (μg/mL)
SOR	5–25	= 7.8097 × 10^4 + 4.6484 × 10^4	1.391 × 10^3	2.3076 × 10^4	0.9990	0.12	0.40
BEN	5–25	= 3.3313 × 10^4 + 1.2421 × 10^4	7.52 × 10^2	1.2469 × 10^4	0.9985	0.10	0.33
CAR	2–10	= 4.3383 × 10^4 + 1.8834 × 10^4	6.86 × 10^2	4.555 × 10^3	0.9992	0.16	0.53
ALU	2–10	= 5.5741 × 10^4  − 7.427 × 10^3	9.22 × 10^2	1.5292 × 10^4	0.9992	0.11	0.35
PON	2–10	= 6.043 × 10^3 + 5.0389 × 10^4	2.150 × 10^3	1.4264 × 10^4	0.9962	0.17	0.56
CAF	5–25	= 2.4885 × 10^4 + 4.6613 × 10^4	4.81 × 10^2	3.1891 × 10^4	0.9989	0.19	0.63

: peak area; : concentration (μg/mL); : standard deviation of the slope; : standard deviation of the intercept.