Nanotechnology-Based Surface Plasmon Resonance Affinity Biosensors for In Vitro Diagnostics

Table 2

Main advantages and drawbacks about bulk SPR, localized SPR, and SPR imaging transduction in ABBs.

	Bulk SPR	Localized SPR	SPR imaging
Advantages
Label-free	∙∙	∙∙∙	∙∙∙
Real-time monitoring	∙∙∙	∙∙∙	∙∙∙
Sensitivity	∙	∙∙	∙∙∙
Multidetection array	—	∙∙	∙∙
Reusability	∙∙	∙∙	∙∙
Being easy to perform	∙∙	∙∙	∙∙
Low sample/ligand volume required	∙∙	∙∙∙	∙∙∙
Drawbacks
Steric hindrance	∙∙	∙	∙
Nonspecific adsorption of proteins	∙∙	∙	∙
Low molecular weight detection	∙∙	∙	∙

(∙) Low, (∙∙) moderate, and (∙∙∙) high.