Selective Sensing of Tyrosine Phosphorylation in Peptides Using Terbium(III) Complexes

Figure 5

Time-dependent change of the luminescence intensity from -L¹ for the phosphorylation of P1 by protein tyrosine kinase Src. At time = 0, Src kinase was added to the solution containing other species and the reaction was started. [Src] = 4 (pink) and 0.4 μg/mL (navy). [P1] = 5 μM, [-L¹] = 100 μM, [ATP] = 5 μM, and [MnCl₂] = 1 mM. The excitation at 262.5 nm and the emission at 545 nm. Reprinted with permission from [69]. Copyright 2014, Springer.