Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil

<div>MRM chromatogram of an extracted soil sample with peaks representing<i> m/z</i> transitions (1) 252 <svg height="6.27169pt" id="M50" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -6.22188 16.9064 6.27169" width="16.9064pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,2.179,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z" id="g117-148"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="954" vert-adv-y="954"></glyph.data></g></svg> 126, (2) 180 <svg height="6.27169pt" id="M51" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -6.22188 16.9064 6.27169" width="16.9064pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,2.179,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z" id="g117-148"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="954" vert-adv-y="954"></glyph.data></g></svg> 72, and (3) 180 <svg height="6.27169pt" id="M52" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -6.22188 16.9064 6.27169" width="16.9064pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,2.179,0)"><path d="M901 255C861 290 790 361 716 442L694 427L796 280H69V230H796L694 83L716 68C790 149 861 220 901 255Z" id="g117-148"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="954" vert-adv-y="954"></glyph.data></g></svg> 84. Peak areas correspond to 0.5 <i>μ</i>M of muramic acid and 15.3 and 1.4 <i>μ</i>M of glucosamine and galactosamine, respectively.</div>

International Journal of Analytical Chemistry

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Figure 4

Figure 4: Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil 

Figure 4 | Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil 