Liquid Chromatography with Electrospray Ionization and Tandem Mass Spectrometry Applied in the Quantitative Analysis of Chitin-Derived Glucosamine for a Rapid Estimation of Fungal Biomass in Soil

Table 3

LC-MS/MS method performance of retention, linearity, sensitivity, precision, limit of detection, and limit of quantification for glucosamine, galactosamine, and muramic acid.

Analyte	transition	(min)	Linearity	Sensitivity	Prec. L/H (%)	LOD (M)	LOQ (M)
Glucosamine	180 > 72	4.41 ± 0.04	0.9997	3.8E4 ± 0.33E4	1.3/1.0	0.025	0.07
Glucosamine	180 > 84	4.41 ± 0.04	0.9997	2.1E4 ± 0.17E4	0.8/0.7	0.05	0.2
Galactosamine	180 > 72	4.52 ± 0.03	0.9999	8.1E4 ± 0.78E4	0.3/0.7	0.01	0.03
Galactosamine	180 > 84	4.52 ± 0.03	0.9997	3.8E3 ± 0.38E3	1.8/1.5	0.5	1.2
Muramic acid	252 > 126	2.01 ± 0.01	0.9994	5.1E4 ± 0.20E4	1.0/0.6	0.01	0.03

Pearson correlation coefficient () determined for seven points, ranging from LOQ to 40 M ().
Regression slope expressed in area units (counts)/M ().
Precision expressed as relative standard deviation (RSD) for repeated injections at 1 M (L) and 10 M (H) ().
Limit of detection reported as the injected concentration giving a peak height corresponding to three times the baseline noise level.
Limit of quantification reported as the injected concentration giving a peak height corresponding to ten times the baseline noise level.
Precision at low level was investigated at a concentration of 2 M for the 180 > 84 transition of galactosamine, due to its relatively high LOD.