Research Article
Arsenic Species Analysis at Trace Level by High Performance Liquid Chromatography with Inductively Coupled Plasma Mass Spectrometry
Table 1
Working conditions of the HPLC and ICP–MS system.
| | Parameters | Value |
| | ICP-MS system | | | RF power (W) | 1500 | | Cool flow (L min−1) | 14.00 | | Auxiliary gas flow (L min−1) | 0.80 | | Nebuliser gas flow (L min−1) | 1.015 | | Collision gas flow (L min−1) | He/4.09 | | CCT1 flow (mL min−1) | 5.198 | | Sampler depth (mm) | 5.0 | | Spray chamber temperature/°C | 2.70 | | Operation mode | KED mode | | Isotopes monitored | 75As | | Dwell time (ms) | 200 | | HPLC system | | | Column | Hamilton PRP–X100 column (250 mm × 4.1 mm i.d., 10 μm) | | Mobile phase | Phase A: 1.25 mmol/L of Na2HPO4 and 11 mmol/L of KH2PO4, pH 6.116 | | | Phase B: 2.5 mmol/L of Na2HPO4 and 22 mmol/L of KH2PO4, pH 6.057 | | Flow–rate (mL/min) | Gradient elution of range 0.6-1.5 mL/min | | HPLC elution program | 0-4.9 min:100% A, 4.9-5.3 min: 100% A to 100% B, 5.3-12 min:100% B
0-4.3 min: 0.6 mL/min, 4.3-4.6 min: 0.6 to 1.0 mL/min, 4.6-4.9 min: 1.0 to 1.5 mL/min, 4.9-12 min: 1.5 mL/min. | | Column temperature (°C) | Room temperature | | Quantitative loop (μL) | 20 μL |
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