Research Article
The Determination of Parkinson’s Drugs in Human Urine by Applying Chemometric Methods
Table 3
Validation parameters for chemometric determination of levodopa and carbidopa using PLS and PCR methods.
| Parameters | Method | Levodopa | Carbidopa |
| (nm) | | 280 nm | 280 nm |
| Correlation Coefficient (R2) | | 0.9997 | 0.9992 |
| PRESS | PLS | 0.004 | 0.002 | PCR | 0.0001 | 0.002 |
| RMSEC | PLS | 4.21x10−3 | 9.42x10−3 | PCR | 6.6x10−4 | 9.42x10−3 |
| LOD (μg/mL) | PLS | 0.052043 | 0.04631 | PCR | 0.04631 | 0.140332 |
| LOQ (μg/mL) | PLS | 0.157706 | 0.052817 | PCR | 0.140332 | 0.160051 |
| Accuracy (% Recovery ± SD) | PLS | 99.18 ± 0.74 | 99.61 ± 0.32 | PCR | 99.18 ± 0.74 | 99.61 ± 0.32 |
| Precision (Reproducibility) | |
| Intraday (% Recovery ± SD) (n:6) | PLS | 99.65 ± 0.95 | 99.97 ± 0.64 | PCR | 99.96 ± 0.75 | 99.98 ± 0.42 |
| Interday (% Recovery ± SD) (n:6) | PLS | 99.75 ± 0.30 | 98.95 ± 0.82 | PCR | 98.98 ± 0.41 | 99.89 ± 0.81 |
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