Simultaneous Determination of Amphenicols and Metabolites in Animal-Derived Foods Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

Table 2

Mass spectrometry conditions and range of relative intensities for each compound.

Analyte	Polarity	Precursor ion (m/z)	Product ion (m/z)	Declustering voltage (V)	Collision voltage (V)
CAP	−	321	257	113	−10
			152	113	−12
CAP-D5	−	326	157	113	−16
TAP	−	354	290	290	−12
			185	164	−10
FF	−	356	185	141	−20
			119	141	−38
FFA	+	248	230	100	10
			130	100	27
FFA-D3	+	251	233	85	10

Quantification ion. CAP, chloramphenicol; FF, florfenicol; FFA, florfenicol amine; TAP, thiamphenicol.