Simultaneous Determination of Amphenicols and Metabolites in Animal-Derived Foods Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
Table 5
Linearity of standard curve, R2, LOD, and LOQ of amphenicols and metabolites in livestock and poultry meat.
Matrix
Analyte
Linearity (μg/kg)
Linear equation
R2
LOD (μg/kg)
LOQ (μg/kg)
Pork
CAP
0.50–50.0
y = 0.05933x + 0.02204
0.9996
0.11
0.36
TAP
0.50–50.0
y = 0.01864x + 0.02831
0.9992
0.12
0.37
FF
0.50–50.0
y = 0.1219x + 0.05289
0.9998
0.03
0.11
FFA
5.00–200.0
y = 0.07707x − 0.00162
0.9991
0.60
2.00
Beef
CAP
0.5–50.0
y = 0.06262x − 0.01198
0.9995
0.05
0.18
TAP
0.5–50.0
y = 0.01741x + 0.006421
0.9991
0.06
0.20
FF
0.5–50.0
y = 0.1233x − 0.09559
0.9992
0.04
0.13
FFA
5.0–200.0
y = 0.06948x − 0.9006
0.9998
1.50
5.00
Lamb
CAP
0.5–50.0
y = 0.05427x + 0.07979
0.9995
0.06
0.21
TAP
0.5–50.0
y = 0.02154x + 0.02831
0.9992
0.06
0.21
FF
0.5–50.0
y = 0.1553x + 0.2424
0.9998
0.04
0.12
FFA
5.0–200.0
y = 0.05435x − 0.1845
0.9992
0.50
5.00
Chicken
CAP
0.5–50.0
y = 0.08144x − 0.1681
0.9991
0.03
0.05
TAP
0.5–50.0
y = 0.03328x − 0.04784
0.9992
0.03
0.11
FF
0.5–50.0
y = 0.1599x − 0.2144
0.9991
0.03
0.11
FFA
5.0–200.0
y = 0.05276x − 0.1651
0.9991
1.00
3.00
CAP, chloramphenicol; FF, florfenicol; FFA, florfenicol amine; LOD, limit of detection; LOQ, limit of quantification; TAP, thiamphenicol.
