Research Article
Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus
Table 4
Ct values under different concentrations of primers and probes.
| | Primer concentration (μM) | 0.05 | 0.1 | 0.15 | 0.2 | 0.25 | 0.3 | 0.35 | 0.4 |
| SARS-CoV-2 NP probe concentration (μM) | 0.05 | 33.24 | 32.97 | 33.34 | 33.22 | 33.08 | 33.20 | 33.04 | 33.18 | 0.10 | 32.29 | 32.26 | 32.33 | 32.28 | 32.39 | 32.20 | 32.20 | 32.22 | 0.15 | 32.01 | 31.86 | 32.15 | 31.98 | 32.17 | 31.94 | 32.06 | 31.97 | 0.20 | 32.50 | 32.09 | 32.00 | 32.02 | 31.98 | 32.20 | 31.75 | 32.11 |
| SARS-CoV-2 ORF probe concentration (μM) | 0.05 | 34.14 | 34.04 | 34.92 | 36.63 | 35.54 | 39.21 | N/A | N/A | 0.10 | 34.20 | 33.21 | 33.84 | 34.98 | 35.39 | 37.09 | 39.82 | N/A | 0.15 | 33.19 | 32.24 | 33.86 | 33.73 | 34.09 | 36.70 | 39.31 | N/A | 0.20 | 33.72 | 32.62 | 35.90 | 35.14 | 34.31 | 35.76 | 39.69 | N/A |
| FluA M probe concentration (μM) | 0.05 | 31.01 | 30.69 | 30.62 | 30.71 | 30.61 | 30.76 | 30.81 | 31.07 | 0.10 | 30.55 | 30.27 | 30.02 | 30.18 | 29.98 | 29.73 | 29.93 | 29.98 | 0.15 | 30.25 | 30.02 | 29.67 | 29.71 | 29.45 | 29.57 | 29.60 | 29.75 | 0.20 | 30.54 | 29.91 | 29.62 | 29.69 | 29.61 | 29.11 | 29.40 | 29.63 |
| FluB NS probe concentration (μM) | 0.05 | 33.68 | 33.29 | 32.66 | 33.20 | 32.33 | 32.88 | 32.47 | 32.60 | 0.10 | 33.07 | 32.77 | 32.54 | 32.72 | 32.67 | 32.21 | 32.25 | 32.76 | 0.15 | 32.97 | 33.38 | 32.46 | 32.53 | 32.01 | 32.53 | 32.33 | 32.60 | 0.20 | 34.03 | 32.55 | 32.63 | 32.68 | 32.62 | 32.98 | 33.02 | 32.87 |
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